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How to use:
Click on an image below, but start with the wild type maps (intracellular and secreted). If a spot
was identified in wildtype, OR in any other strain, it was annotated on the wildtype map assuming
the spot was present in wildtype. Only spots unique to other strains are annotated in the other maps
(thus the remaining maps have very few spots noted). To get the relevant information, mouse over the
spot of interest. That will bring up a unique code (e.g. R20). Click anywhere on the map to open a
pdf file (requires Adobe 5.0 or higher). Search the pdf file for the code to identify the protein.
Note that in some cases a single spot may be related to multiple proteins.
'ACC#' refers to an in-house database at Cornell.
'PSPTO' refers to the RefSeq identifier.
'NCBI ACC#' refers to an NCBInr entry and 'protein name' refers to the associated NCBI name.
Spots were visualized with Sypro Ruby on a Fuji FLA3000 scanner. Plugs were analyzed by MS and
MSMS combined searches using data obtained on an AB4700 TOF/TOF. GPS Explorer hits at confidence >95%
(version 3 software) were accepted. The highest MOWSE score achieved to date has been 1030.
For any inquiries, feel free to send us an email.
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