Recent Publications

If a journal would like us to remove a pdf archive, please send email to khl9 at cornell.edu

Gupta P, Lee KH (2007). "Genomics and Proteomics in Process Development: Opportunities and Challenges". Trends in Biotechnology. Accepted.

KH Lee, NR Relkin (2007). "Reply: Cerebrospinal fluid biomarkers for Alzheimer's disease". In press Annals of Neurology. In press.

DH Lee, SG Kim, YC Park, SW Nam, KH Lee and JH Seo (2007). "Proteome analysis of recombinant Escherichia coli producing human glucagon-like peptide-1". Journal of Chromatography B. In press. doi:10.1016/j.jchromb.2006.09.042

EJ Finehout, Z Franck, LH Choe, NR Relkin and KH Lee (2007). "Cerebrospinal Fluid Proteomic Biomarkers for Alzheimer's Disease". Annals of Neurology. 61: 120-129. pdf here.

The paper above has received significant attention from the media. There is more information available about this paper at various sites:
Cornell press release.
Link to supplementary figures for media use.
Cornell Chronicle story on this.
The CBS Evening News did a 3+ minute segment on this. I have a copy of the video for noncommercial use if you ask.
Other CBS Evening News links here and here.
ScienCentral did a piece on this which includes video (video access requires signing in).
A general google search based on "cornell 23 alzheimer test". Variations to search for tidbits that you can search for: "Finehout", "spinal fluid", "proteomics"
WebMD did a nice piece on this.
Time magazine blog.
More stories and links from Newswise and CTV Canada and Wall Street Journal (requires paid access, but another free version is available here).

EJ Finehout, Z Franck, NR Relkin and KH Lee (2006). "Proteomic Analysis of Cerebrospinal Fluid Changes Related to Postmortem Interval". Clinical Chemistry. 52: 1906-1913. pdf here.

KH Lee (2006). "Editorial". Briefings in Functional Genomics and Proteomics 5: 97. pdf here.

K Aggarwal, LH Choe and KH Lee (2006). "Shotgun proteomics using the iTRAQ isobaric tags". Briefings in Functional Genomics and Proteomics 5: 112-120. pdf here.

LH Choe, B Werner, and KH Lee (2006). "Two-dimensional Protein Electrophoresis: From Molecular Pathway Discovery to Biomarker Discovery in Neurological Disorders". NeuroRX 3: 327-335. pdf here.

R Kuczenski, K Aggarwal, and KH Lee (2005). "Improved Understanding of Gene Expression Regulation Using Systems Biology". Expert Review in Proteomics 2: 915-924. pdf here.

M D'Ascenzo, NR Relkin, and KH Lee (2005). "Alzheimer's Disease Cerebrospinal Fluid Biomarker Discovery: A Proteomics Approach". Current Opinion in Molecular Therapeutics 7: 557-564. No pdf available.

J Diao, D Ren, JR Engstrom, and KH Lee (2005). "A Surface Modification Strategy on Silicon Nitride for Developing Biosensors". Analytical Biochemistry 343: 322-328. pdf here.

Y Yang, C Li, KH Lee, and HG Craighead (2005). "A Polymeric Microchip with Integrated Tips and in situ Polymerized Monolith for Electrospray Mass Spectrometry". Lab on a Chip 5: 869-876. pdf here.

Y Yang, C Li, KH Lee, and HG Craighead (2005). "Coupling On-Chip Solid Phase Extraction to Electrospray Mass Spectrometry Through an Integrated Electrospray Tip". Electrophoresis 26: in press. pdf here.

LH Choe, K Aggarwal, Z Franck and KH Lee (2005). "A Comparison of the Consistency of Proteome Quantitation Using Two-Dimensional Electrophoresis and Shotgun Isobaric Tagging in Escherichia coli Cells". Electrophoresis. 26: 2437-2449. pdf here.

EJ Finehout, Z Franck and KH Lee (2005). "Complement Protein Isoforms in CSF as Possible Biomarkers for Neurodegenerative Disease". Disease Markers. 21: 93-101. pdf here.

E Finehout, J Cantor and KH Lee (2005). "Kinetic Characterization of Sequencing Grade Modified Trypsin". Proteomics. 5: 2319-2321. pdf here.

C Li, Y Yang, HG Craighead and KH Lee (2005). "Isoelectric Focusing in Cyclic Olefin Copolymer Microfluidic Channels Coated by Polyacrylamide Using a UV Photografting Method". Electrophoresis. 26: 1800-1806. pdf here.

K Aggarwal, LH Choe and KH Lee (2005). "Quantitative Analysis of Protein Expression Using Amine-Specific Isobaric Tags in Escherichia coli Cells Expressing rhsA Elements". Proteomics. 5: 2297-2308. pdf here.

EJ Hayduk and KH Lee (2005). "Cytochalasin D Improves Heterologous Protein Productivity in Adherent Chinese hamster ovary cells". Biotechnology and Bioengineering. 90: 354-364. pdf here.

PS Lee and KH Lee (2005). "Engineering HlyA Hypersecretion in Escherichia coli Based on Proteomic and Microarray Analyses". Biotechnology and Bioengineering 89: 195-205. pdf here.

KS Brown, CC Hill, GA Calero, C Myers, KH Lee, JP Sethna and RA Cerione (2004). "The Statistical Mechanics of Sloppy Models: Nerve Growth Factor Signaling". Phys. Biol. 1: 184-195. pdf here.

YC Park, SG Kim, KH Lee, K Park, and JH Seo, "Fed-batch production of D-ribose from sugar mixtures by transketolase deficient Bacillus subtilis SPK1" Applied Microbiology and Biotechnology 66: 297-302. pdf here.

C Li and KH Lee, "Microfluidic Device for Affinity Removal of Albumin from Human Cerebrospinal Fluid" Analytical Biochemistry (2004). pdf here.

LB Shaw, JP Sethna and KH Lee, "Mean field approaches to the totally asymmetric exclusion process with quenched disorder and large particles." Physics Review E. 70: 021901 (2004). pdf here.

E Hayduk, LH Choe and KH Lee, "A Two-dimensional Electrophoresis Map of Chinese Hamster Ovary Cell Proteins Based on Fluorescence Staining" Electrophoresis. 25: 2545-2556 (2004). pdf here.

EJ Finehout, Z Franck, and KH Lee, "Towards two-dimensional electrophoresis mapping of the cerebrospinal fluid proteome from a single individual" Electrophoresis. 25: 2564-2575 (2004). pdf here.

EJ Finehout and KH Lee, "An Introduction to Mass Spectrometry Applications in Biological Research". Biochemistry and Molecular Biology Education. 32: 93-100 (2004). pdf here.

KH Lee and KF Reardon, "Proteomics: An Exciting New Science, but Where are the Chemical Engineers?" AIChE Journal. 49: 2682-2686 (2003). pdf here.

LB Shaw, A Kolomeisky and KH Lee, "Local Inhomogeneity in Asymmetric Simple Exclusion Processes with Extended Objects". Journal of Physics A Mathematical and General 37: 2105-2113 (2004). pdf here.

WC Lee and KH Lee, "Applications of Affinity Chromatography in Proteomics". Analytical Biochemistry. 3241-10 (2004). pdf here.

A Mehra, KH Lee and V Hatzimanikatis, "Insights into the Relation Between mRNA and Protein Expression Patterns. I. Theoretical Considerations". Biotechnology and Bioengineering. 84: 822-833 (2003). pdf here.

PS Lee, LB Shaw, LH Choe, A Mehra, V Hatzimanikatis and KH Lee, "Insights into the Relation Between mRNA and Protein Expression Patterns. II. Experimental Observations in Escherichia coli". Biotechnology and Bioengineering. 84: 834-841 (2003). pdf here.

PS Lee and KH Lee, "Escherichia coli-a Model System That Benefits From and Contributes to the Evolution of Proteomics". Biotechnology and Bioengineering. 84: 801-814 (2003). pdf here.

EJ Finehout and KH Lee, "A Comparison of Automated In-Gel Digest Methods for Femtomole Level Samples". Electrophoresis. 24: 3508-3516 (2003). pdf here.

LH Choe and KH Lee, "A Quantitative and Qualitative Measure of Intralaboratory Two-dimensional Gel Reproducibility and the Effects of Sample Preparation, Sample Load, and Image Analysis". Electrophoresis. 24: 3500-3507 (2003). pdf here.

K Aggarwal and KH Lee, "Functional Genomics and Proteomics as a Foundation for Systems Biology". Briefings in Functional Genomics and Proteomics, 2: 175-184 (2003). pdf here.

LB Shaw, R Zia and KH Lee, "Modeling, Simulations and Analyses of Protein Synthesis: a Driven Lattice Gas with Extended Objects". Physical Review E 68: 021910 (2003). pdf here.

C Li, WC Lee, and KH Lee, "Affinity Separations Using Microfabricated Microfluidic Devices: in situ Photopolymerization and Use in Protein Separations". Biotechnology and Bioprocess Engineering. 8: 240-245 (2003). pdf here.

E Hayduk, LH Choe and KH Lee, "Proteomics as a Tool in Discovery-Based Science". Current Science 83: 840-844 (2003).

Choe, L.H., Dutt, M.J., Relkin, N. and K.H. Lee, "Studies of Potential Cerebrospinal Fluid Molecular Markers for Alzheimer's Disease". Electrophoresis 23: 2247-2251 (2002). pdf here.

Choe, L.H., Green, A., Knight, R.S.G., Thompson, E.J. and K.H. Lee, "Apolipoprotein E and Other Cerebrospinal Fluid Proteins Differentiate Antemortem Variant Creutzfeldt-Jakob Disease from Antemortem Sporadic Creutzfeldt-Jakob Disease". Electrophoresis 23: 2242-2246 (2002). pdf here.

Reardon, K.F., K.H. Lee, Wittrup, K.D. and V. Hatzimanikatis "Jay Bailey as Mentor - The Students' Perspective". Biotechnology and Bioengineering 79: 484-489 (2002). pdf here.

Dutt, M.J. and K.H. Lee, "The Scaled Volume as an Image Analysis Variable for Detecting Changes in Protein Expression Levels by Silver Stain". Electrophoresis 22: 1627-1632 (2001). pdf here.

K.H. Lee, "Proteomics: A Technology-Driven and a Technology-Limited Discovery Science". Trends in Biotechnology 19: 217-222 (2001). pdf here.
Reprinted from Trends in Biotechnology with permission from Elsevier Science. Single copies of this article can be downloaded and printed for personal research and study purposes only.

Chen, W., F. Bruhlmann, K.H. Lee, M. Deschusses, "Whole Cell Catalysis". In Encyclopedia of Catalysis, I Horvath, ed. New York: Wiley (2001).

Hatzimanikatis, V., Collins, D., Lawrence, S., Browning, S. and K.H. Lee,"Bioinformatics, Genomics and the Chemical Engineer - a Perspective". Chemical Engineering Education 34: 346-349 (2000).

Dutt, M.J. and K.H. Lee, "Proteomic Analysis". Current Opinion in Biotechnology, 11: 176-179 (2000). pdf here.

Lee, P.S. and K.H. Lee, "Genomic Analysis". Current Opinion in Biotechnology, 11: 171-175 (2000). pdf here.

Choe, L.H. and K.H. Lee, "A Comparison of Three Commercially Available Isoelectric Focusing Units for Proteome Analysis: the Multiphor,the IPGphor and the Protean IEF Cell". Electrophoresis, 21: 993-1000 (2000). pdf here.

Hatzimanikatis, V., K.H. Lee, J.E. Bailey, "A Mathematical Description of Regulation of the G1-S Transition of the Mammalian Cell Cycle". Biotechnology and Bioengineering, 65: 631-637 (1999). pdf here.

Choe, L.H., W. Chen, K.H. Lee, "Proteome Analysis of Fis Overexpression in E. coli". Electrophoresis, 20: 798-805 (1999). pdf here.

Hatzimanikatis, V., L.H. Choe, K.H. Lee, "Proteomics: Theoretical and Experimental Considerations". Biotechnology Progress, 15:312-318 (1999). pdf here.

Hatzimanikatis, V., K.H. Lee, "Dynamical Analysis of Gene Networks Requires Both mRNA and Protein Expression Information". Metabolic Engineering, 1: 275-281 (E1-E7) (1999). pdf here.

Lee, K.H. and M.G. Harrington, "Double-Label Analysis". In 2-D Proteome Analysis Protocols, A Link, ed. New York: Humana Press, Pages 291-295 (1998).

Lee, K.H. and M.G. Harrington, "Preparative Gel Electrophoresis". In Gel Electrophoresis of Proteins, BD Hames, ed. Oxford: Oxford University Press, Pages 93-104 (1998).

Lee, K.H. and M.G. Harrington, "The Assay Development of a Molecular Marker for Transmissible Spongiform Encephalopathies". Electrophoresis 18: 502-506 (1997).

Lee, K.H. and M.G. Harrington, "14-3-3 and BSE". Vet. Record. 140: 206-207 (1997).

Lee, K.H., A. Sburlati, W. Renner, J.E. Bailey, "Deregulated Expression of Cloned Transcription Factor E2F-1 in Chinese Hamster Ovary Cells Shifts Protein Patterns and Activates Growth in Protein-Free Medium". Biotech. Bioeng., 50: 273-279 (1996).

Lee, K.H., M.G. Harrington, and J.E. Bailey, "Two-Dimensional Electrophoresis of Proteins as a Tool in the Metabolic Engineering of Cell Cycle Regulation". Biotech. Bioeng. 50: 336-340 (1996).

Lee, K.H. and M.G. Harrington, "Premortem Diagnosis of Creutzfeldt-Jakob Disease by Cerebrospinal Fluid Analysis". The Lancet, 348: 887 (1996).

Will, R.G., Zeidler, M., Brown, P., Harrington, M.G., Lee, K.H. and Kenney, K., "Cerebrospinal fluid test for new variant Creutzfeldt-Jakob disease". The Lancet, 348: 955 (1996).

Hsich, G., Kenney, K., Gibbs, C.J., Lee, K.H. and Harrington, "The 14-3-3 brain protein in cerebrospinal fluid as a marker for transmissible spongiform encephalopathies". The New England Journal of Medicine, 335: 924-930 (1996).