Mascot Search Results

Pseudomonas syringae TOF-TOF Example

Search title      : SampleSetID: 2022, AnalysisID: 4593, MaldiWellID: 58508, SpectrumID: 69836, 
                           Path=\LC PS 2003\ps 2003-10-02\c B16 1\1\B16
Database          : syringae6frame new (1836856 sequences; 298367028 residues)
Taxonomy        : Bacteria (Eubacteria) (1836856 sequences)
Timestamp       : 3 Oct 2003 at 17:57:19 GMT
Significant hits : 16853.00116675M 4887159..4888607

Probability Based Mowse Score

Score is -10*Log(P), where P is the probability that the observed match is a random event.
Individual ions scores > 34 indicate identity or extensive homology (p<0.05).

Score Distribution

Peptide Summary Report

Switch to Protein Summary Report

1. 16853.00116675M  Mass: 52013   Total score: 752  Peptides matched: 14

   4887159..4888607

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	Query Observed Mr(expt) Mr(calc) Delta Miss Score Rank Peptide
	11 962.58 961.57 961.56 0.01 0 42 1 ILHVGDPLV
	18 1257.73 1256.72 1256.71 0.01 0 96 1 FVALLGDLQGPK
	22 1360.72 1359.71 1359.69 0.02 0 59 1 SSVPIYAFSPHR
	38 1598.85 1597.84 1597.83 0.01 0 36 1 AEAVANDEVLDALIR
	41 1629.90 1628.89 1628.88 0.02 1 16 1 IRSSVPIYAFSPHR
	44 1698.91 1697.90 1697.88 0.02 0 35 1 GDLGVEIGDAELVGVQK
	49 1826.91 1825.90 1825.89 0.01 0 79 1 LAAEMDLDYLAVSFPR + Oxidation (M)
	54 1852.00 1850.99 1850.98 0.01 0 18 1 AIIALTESGYTPLIMSR + Oxidation (M)
	58 1997.08 1996.07 1996.06 0.01 1 10 3 IERAEAVANDEVLDALIR
	65 2528.26 2527.25 2527.25 0.00 0 45 1 VDTQTAHELHCTVLIGGPLSDHK + Carbamidomethyl (C)
	67 2762.52 2761.51 2761.52 -0.01 0 136 1 IVATLGPASNSPEVIEQLILSGLDVAR
	72 2848.46 2847.45 2847.44 0.00 0 71 1 FTFSTAHPLTSGNQQIVGIDYPDLVK
	73 2924.53 2923.52 2923.54 -0.02 1 (53) 1 GVYTVPFDPGALPPGQVSQAAVDELLKR
	75 2924.53 2923.52 2923.54 -0.02 1 108 1 GVYTVPFDPGALPPGQVSQAAVDELLKR

Proteins matching the same set of peptides:

16853.00116675   Mass: 51882   Total score: 752  Peptides matched: 14 
   4887162..4888607

16853.00116674M  Mass: 52247   Total score: 752  Peptides matched: 14 
   4887153..4888607

16853.00116674   Mass: 52116   Total score: 752  Peptides matched: 14 
   4887156..4888607

2. 16853.00559896   Mass: 17186   Total score: 24  Peptides matched: 1

  complement(1950084..1950542)

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	Query Observed Mr(expt) Mr(calc) Delta Miss Score Rank Peptide
	38 1598.85 1597.84 1597.86 -0.02 1 24 2 IDAVTQPGGWRTIGK

Proteins matching the same set of peptides:

16853.00559896M  Total score: 24  Peptides matched: 1  
16853.00559895   Total score: 24  Peptides matched: 1  
16853.00559895M  Total score: 24  Peptides matched: 1

3. 16853.00361098   Mass: 113791   Total score: 23  Peptides matched: 1

   2338769..2341834

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	Query Observed Mr(expt) Mr(calc) Delta Miss Score Rank Peptide
	38 1598.85 1597.84 1597.84 0.00 1 23 3 NQMRVNISGLDVPR

Proteins matching the same set of peptides:

16853.00361098M  Total score: 23  Peptides matched: 1

4. 16853.00810503M  Mass: 33810   Total score: 22  Peptides matched: 1

   complement(3798230..3799168)

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	Query Observed Mr(expt) Mr(calc) Delta Miss Score Rank Peptide
	18 1257.73 1256.72 1256.68 0.04 1 22 2 GWGGWLAVGARK

Proteins matching the same set of peptides:

16853.00810503   Total score: 22  Peptides matched: 1  
16853.00810504M  Total score: 22  Peptides matched: 1  
16853.00810504   Total score: 22  Peptides matched: 1  
16853.00810505M  Total score: 22  Peptides matched: 1  
16853.00810505   Total score: 22  Peptides matched: 1  
16853.00810506M  Total score: 22  Peptides matched: 1  
16853.00810506   Total score: 22  Peptides matched: 1

5. 16853.00294681M  Mass: 31819   Total score: 22  Peptides matched: 1

   6020521..6021405

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	Query Observed Mr(expt) Mr(calc) Delta Miss Score Rank Peptide
	18 1257.73 1256.72 1256.75 -0.03 0 22 3 MVLAEAILLLR + Oxidation (M)

Unassigned queries: (no details means no match)

	Query Observed Mr(expt) Mr(calc) Delta Miss Score Rank Peptide
	58 1997.08 1996.07 1996.05 0.03 1 12 1 MYYRLDVVNLELSPLR + Oxidation (M)
	15 1057.43 1056.42 1056.46 -0.04 1 7 1 PGCCDHRK + 2 Propionamide (C)

 
Search  Parameters:

Type of search                  : MS/MS Ion Search
Enzyme                             : Trypsin
Variable modifications       : Carbamidomethyl (C),Oxidation (M),Propionamide (C)
Mass values                      : MONOISOTOPIC
Protein Mass                     : Unrestricted
Peptide Mass Tolerance    : ± 50 ppm
Fragment Mass Tolerance : ± 0.3 Da
Max Missed Cleavages      : 1
Instrument type                : MALDI-TOF-TOF
Number of queries             : 80