Mascot Search Results

Pseudomonas syringae TOF-TOF Example

Search title : SampleSetID: 2022, AnalysisID: 4593, MaldiWellID: 58508, SpectrumID: 69836, Path=\LC PS 2003\ps 2003-10-02\c B16 1\1\B16
Database : syringae6frame new (1836856 sequences; 298367028 residues)
Taxonomy : Bacteria (Eubacteria) (1836856 sequences)
Timestamp : 3 Oct 2003 at 17:57:19 GMT
Significant hits: 16853.00116675M 4887159..4888607

Probability Based Mowse Score

Score is -10*Log(P), where P is the probability that the observed match is a random event.
Individual ions scores > 34 indicate identity or extensive homology (p<0.05).

Peptide Summary Report

Switch to Protein Summary Report

1. 16853.00116675M Mass: 52013 Total score: 752 Peptides matched: 14 
 4887159..4888607
Check to include this hit in error tolerant search or archive report

Query   Observed   Mr(expt)   Mr(calc)   Delta  Miss Score  Rank   Peptide

11 962.58     961.57     961.56     0.01    0    42     1    ILHVGDPLV

18 1257.73    1256.72    1256.71     0.01    0    96     1    FVALLGDLQGPK

  22    1360.72    1359.71    1359.69     0.02    0    59     1    SSVPIYAFSPHR

  38    1598.85    1597.84    1597.83     0.01    0    36     1    AEAVANDEVLDALIR

  41    1629.90    1628.89    1628.88     0.02    1    16     1    IRSSVPIYAFSPHR

  44    1698.91    1697.90    1697.88     0.02    0    35     1    GDLGVEIGDAELVGVQK

  49    1826.91    1825.90    1825.89     0.01    0    79     1    LAAEMDLDYLAVSFPR + Oxidation (M)

  54    1852.00    1850.99    1850.98     0.01    0    18     1    AIIALTESGYTPLIMSR + Oxidation (M)

  58    1997.08    1996.07    1996.06     0.01    1    10     3    IERAEAVANDEVLDALIR

  65    2528.26    2527.25    2527.25     0.00    0    45     1    VDTQTAHELHCTVLIGGPLSDHK + Carbamidomethyl (C)

  67    2762.52    2761.51    2761.52    -0.01    0   136     1    IVATLGPASNSPEVIEQLILSGLDVAR

  72    2848.46    2847.45    2847.44     0.00    0    71     1    FTFSTAHPLTSGNQQIVGIDYPDLVK

  73    2924.53    2923.52    2923.54    -0.02    1   (53)    1    GVYTVPFDPGALPPGQVSQAAVDELLKR

  75    2924.53    2923.52    2923.54    -0.02    1   108     1    GVYTVPFDPGALPPGQVSQAAVDELLKR

Proteins matching the same set of peptides:
16853.00116675 Mass: 51882 Total score: 752 Peptides matched: 14 
 4887162..4888607
16853.00116674M Mass: 52247 Total score: 752 Peptides matched: 14 
 4887153..4888607
16853.00116674 Mass: 52116 Total score: 752 Peptides matched: 14 
  4887156..4888607
 
 
 
 




 
 
 
 
 
2. 16853.00559896 Mass: 17186 Total score: 24 Peptides matched: 1 
 complement(1950084..1950542)
Check to include this hit in error tolerant search or archive report

Query   Observed   Mr(expt)   Mr(calc)   Delta  Miss Score  Rank   Peptide

  38    1598.85    1597.84    1597.86    -0.02    1    24     2    IDAVTQPGGWRTIGK

Proteins matching the same set of peptides:
16853.00559896M Total score: 24 Peptides matched: 1  
16853.00559895 Total score: 24 Peptides matched: 1 
16853.00559895M Total score: 24 Peptides matched: 1 
 
 
 
 




 
 
 
 
 
3. 16853.00361098 Mass: 113791 Total score: 23 Peptides matched: 1 
 2338769..2341834
Check to include this hit in error tolerant search or archive report

Query   Observed   Mr(expt)   Mr(calc)   Delta  Miss Score  Rank   Peptide

  38    1598.85    1597.84    1597.84     0.00    1    23     3    NQMRVNISGLDVPR

Proteins matching the same set of peptides:
16853.00361098M Total score: 23 Peptides matched: 1 
 
 
 
 




 
 
 
 
 
4. 16853.00810503M Mass: 33810 Total score: 22 Peptides matched: 1 
 complement(3798230..3799168)
Check to include this hit in error tolerant search or archive report

Query   Observed   Mr(expt)   Mr(calc)   Delta  Miss Score  Rank   Peptide

18    1257.73    1256.72    1256.68     0.04    1    22     2    GWGGWLAVGARK

Proteins matching the same set of peptides:
16853.00810503 Total score: 22 Peptides matched: 1 
16853.00810504M Total score: 22 Peptides matched: 1 
16853.00810504 Total score: 22 Peptides matched: 1 
16853.00810505M Total score: 22 Peptides matched: 1 
16853.00810505 Total score: 22 Peptides matched: 1 
16853.00810506M Total score: 22 Peptides matched: 1 
16853.00810506 Total score: 22 Peptides matched: 1 
 
 
 
 




 
 
 
 
 
5. 16853.00294681M Mass: 31819 Total score: 22 Peptides matched: 1 
 6020521..6021405
Check to include this hit in error tolerant search or archive report

Query   Observed   Mr(expt)   Mr(calc)   Delta  Miss Score  Rank   Peptide

18    1257.73    1256.72    1256.75    -0.03    0    22     3    MVLAEAILLLR + Oxidation (M)

 
 
 




 
 
 
 
 
 
 
 
 




 
 
 
 
 
Unassigned queries: (no details means no match) 

Query   Observed   Mr(expt)   Mr(calc)   Delta  Miss Score  Rank   Peptide

58 1997.08    1996.07    1996.05     0.03    1    12     1    MYYRLDVVNLELSPLR + Oxidation (M)

15  1057.43    1056.42    1056.46    -0.04    1     7     1    PGCCDHRK + 2 Propionamide (C)

Search Parameters

Type of search : MS/MS Ion Search
Enzyme : Trypsin
Variable modifications : Carbamidomethyl (C),Oxidation (M),Propionamide (C)
Mass values : MONOISOTOPIC
Protein Mass : Unrestricted
Peptide Mass Tolerance : ± 50 ppm
Fragment Mass Tolerance: ± 0.3 Da
Max Missed Cleavages : 1
Instrument type : MALDI-TOF-TOF
Number of queries : 80

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